Z730033
Sigma
Gene Transfer: Delivery and Expression of DNA and RNA, A Laboratory Manual
| ISBN-10: | 0-87969-765-2 |
| ISBN-13: | 978-0-87969-765-5 |
Description
| General description | Understanding gene function and regulation requires rigorous testing in live cells and organisms. Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing their expression, as well as new clinical applications that rely upon the introduction of genetic material. The vast number of available techniques for clinical and laboratory research often makes selecting the optimal method a difficult process. This book provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. The detailed, step-by-step protocols include viral and nonviral methods of gene delivery, transgenic approaches, strategies for the regulation of transgene expression, and modification of the host response. |
Properties
| publication info | T. Friedmann and J. Rossi, ed., Cold Spring Harbor Laboratory Press, 2006, 793 pp., soft cover |
Table Of Contents
| Table of Contents | PARTIAL TOC 1. Introduction VIRAL VECTORS 2. Retroviral Vectors 3. Development of Lentiviral Vectors Expressing siRNA 4. HIV–2 Vectors in Human Gene Therapy: Design, Construction, and Therapeutic Potential 5. SIV Vectors as Vehicles for DNA Delivery 6. Production and Use of Feline Immunodeficiency Virus–based Lentiviral Vectors 7. Lentivirus Transduction of Hematopoietic Cells 8. Spleen Necrosis Virus–based Vectors 9. Foamy Virus Vector Production and Transduction of Hematopoietic Cells 10. Simian Foamy Virus Type–1 Vectors 11. Generation of VSV–G–pseudotyped Retroviral Vectors 12. Targeted Gene Transfer with Surface–engineered Lentiviral Vectors 13. Preparation of Pseudotyped Lentiviral Vectors Resistant to Inactivation by Serum Complement 14. Generation of 2A Peptide–linked Multicistronic Vectors 15. Construction of First–generation Adenoviral Vectors 16. Production and Characterization of Helper–dependent Adenoviral Vectors 17. Cell and Tissue Targeting 18. Stable Producer Cell Lines for AAV Assembly 19. Strategies for the Design of Hybrid Adeno–associated Virus Vectors 20. Recombinant Herpes Simplex Virus Vectors 21. Herpes Simplex Virus Type–1–derived Amplicon Vectors 22. γ–2 Herpesvirus Saimiri–based Vectors 23. Gene Delivery Using HSV/AAV Hybrid Amplicon Vectors 24. Polyomaviruses: SV40 25. 26. 27. 28. 29. 30. 31. 32. 33. NONVIRAL TECHNIQUES AND VECTORS 34. An Overview of Condensing and Noncondensing Polymeric Systems for Gene Delivery 35. Transfection of Hippocampal Neurons with Plasmid DNA Using Calcium Phosphate Coprecipitation 36. Gene Delivery to Skin Using Biolistics 37. Optimizing Electrotransfection of Mammalian Cells In Vitro 38. Micro In Utero Electroporation for Efficient Gene Targeting in Mouse Embryos 39. Lipoplex and LPD Nanoparticles for In Vivo Gene Delivery 40. Bioresponsive Targeted Charge Neutral Lipid Vesicles for Systemic Gene Delivery 41. HVJ Liposomes and HVJ Envelope Vectors 42. Polylysine Copolymers for Gene Delivery 43. PEI Nanoparticles for Targeted Gene Delivery 44. Cyclodextrin–containing Polycations for Nucleic Acid Delivery 45. Bionanocapsules Using the Hepatitis B Virus Envelope L Protein 46. Formulations of Solid Lipid Nanoparticles for Transfection of Mammalian Cells In Vitro 47. PEGylated Poly–l–lysine DNA Nanoparticles 48. Water–soluble Lipopolymers and Lipopeptides for Nucleic Acid Delivery 49. Cationic Polysaccharides for DNA Delivery 50. 51. 52. 53. 54. 55. 56. 57. 58. 59. REGULATION OF TRANSGENE EXPRESSION 60. Conditional Mutagenesis of the Genome Using Site–specific DNA Recombination 61. Expression and Validation of Ribozyme and Short Hairpin RNA in Mammalian Cells 62. Mifepristone–inducible Gene Regulatory System 63. Dimerizer–mediated Regulation of Gene Expression 64. 65. 66. SPECIALIZED TECHNIQUES OF GENE AND VECTOR DELIVERY 67. Assembly of De Novo Bacterial Artificial Chromosome–based Human Artificial Chromosomes 68. Delivery of Naked DNA Using Hydrodynamic Injection Techniques 69. Nonviral Gene Transfer across the Blood–brain Barrier with Trojan Horse Liposomes 70. 71. 72. 73. TRANSGENIC APPROACHES 74. Pronuclear Microinjection in Mice 75. Knockdown Transgenic Mice Generated by Silencing Lentiviral Vectors APPENDIX |
